Active substances: Norfloxacin
Paul, MN. An ophthalmic, preservative-free formulation of ciprofloxacin 0. Bacterial strains of S.
Synthesis of Nanosphere-EncapsulatedCiprofloxacin Synthesis of the pullulan-polycaprolactone nanoparticles was performed as previously described. Both solutions were precipitated with methanol and dried under a vacuum.
Next, hydrolyzed pullulan was dissolved in NMP, 4-dimethylamino pyridine was added with the PCL nitrophenyl carbonate, the mixture was stirred for 48 hours, and the solution was precipitated with ethanol.
After a toluene wash, the solid residue was dried for 24 hours under vacuum.
To prepare the nanospheres, 100 mg of block copolymer was dissolved in 2 mL of dimethyl sulfoxide DMSO, along with 15 mg of the free-base ciprofloxacin. Deionized water 10 mL was then added drop-wise during constant stirring to form the micelles.
To remove the DMSO, the solution was placed in dialysis cassettes and submerged in water that contained 1. As presented in Fig.
Treatment with the lowest concentration of ciprofloxacin 0. By contrast, cells treated with higher concentrations of ciprofloxacin 0.
A decrease in the number of cells, as well as a loss in cell-cell contact was also observed.
The effect of ciprofloxacin on cellular GSH levels There is a strong correlation between cellular GSH depletion and the progression of apoptosis 22.
The exposure of cells to lower concentrations of ciprofloxacin 0. According to the quantitative analysis of image cytometry data Fig.
Graphs are representative of three independent experiments with similar results.