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In the process, a host cell eg, a yeast or fungus, into which has been introduced both a nucleic acid encoding a transcriptional regulator fusion protein of the methods of the invention and a nucleic acid encoding the protein of the interest operatively linked to at least one tet operator sequence, is grown at production scale in a culture medium in the presence of a substituted tetracycline compound to stimulate transcription of the nucleotides sequence encoding the protein of interest ie, the nucleotide sequence operatively linked to the tet operator sequence s and the protein of interest is isolated from harvested host cells or from the culture medium.
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