Active substances: Doxycycline
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Transfer of a Grant. Materials and Methods For the present study, 15 single rooted anterior teeth indicated for extraction due to advanced chronic periodontitis were selected from the outpatient department, Department of Periodontology and Oral Implantology.
Freshly extracted single rooted human teeth with proximal attachment loss of 5 mm or more, no history of root planing or prophylaxis in the past 6 months, no history of acute pain or swelling, absence of any restoration or caries were selected. Preparation of the specimen A total of 45 dentin samples were obtained from the periodontally affected mid root region of 15 extracted teeth Following extraction, the teeth were washed and cleaned using a soft bristle brush and stored in distilled water Scaling and root planing of root surfaces was done to obtain smooth, shiny hard surface Samples were obtained from the cervical two thirds of the root by making two parallel grooves 0.