Active substances: Ciprofloxacin
In fact, IL-6 is considered as a treatment target in IBD and other inflammatory conditions,. Other major inflammatory cytokines such as KC and Rantes are also known to induce serious tissue damage, if their levels are too high and meet no resolution,.
IL-3 is an essential mediator for myeloid lineage development and has been reported to be elevated by CIP treatment.
Analysis on cell-death type-specific markers revealed that after CI villous epithelial cells underwent apoptosis Figure 6 A while crypt cells displayed elevated formation of autophagosomes Figure 6 B. CIP effectively reduced both types of cell death.
A high level of IgA coated the ileal mucosa of CI-mice, particularly in foci exhibiting severe morphological damage in villous tips. This finding correlates with observations found in human T cells in vitro, and in CD 2 F 1 mice.
Similarly, the autophagic death, which occurred in crypt cells of CI-mice in our study, was also found in CD 2 F 1 mice. In summary, CIP significantly increased survival, altered the serum cytokine and chemokine profile, accelerated bone-marrow recovery, inhibited cell death of ileum, and prevented systemic bacterial infection.
Euthanasia was carried out in accordance with the recommendations and guidelines of the American Veterinary Medical Association.
Male mice were not used in this study because of problems associated with aggression, which in these experiments could lead to further damage to wound sites and enhanced infection. All mice were randomly assigned to experimental groups.
No more than 4 mice were housed per filter-topped polycarbonate cage MicroIsolator in conventional holding rooms.
Four or five days prior to the experiments, body weights were measured and hair of the dorsal surface was removed using electric clippers. On the day of experiments, mice were first irradiated and then wounded under anesthesia by methoxyflurane inhalation.
All mice, including controls, received an intraperitoneal injection of 0.
After injuries mice were assigned to clean cages and provided with proper food and acidified water. Radiation Injury RI Mice were placed in well-ventilated acrylic restrainers and given 9.
Calibration of the dose rate with alanine was traceable to the National Institute of Standards and Technology and the National Physics Laboratory of the United Kingdom. Sham-irradiated mice were placed in the same acrylic restrainers, taken to the radiation facility, and restrained for the time required for irradiation.