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However, the significance of rivers as environments providing irrigation water and recreational activities as well as the possibility of the potential spread of pathogenic and antibiotic resistant bacteria between the environment and humans and animals, marks them as highly relevant study subjects Czekalski et al.
In addition, anthropogenic activities might impact on water environment promoting the dissemination of antibiotic resistance Pruden et al. Currently, the river is being contaminated by wastewater from urban municipal sewers, industrial activities, and livestock farms.
The objectives of this study were to evaluate the quality of the water between sample locations in the river polluted by different human activities, to determine the presence of pathogenic E.
Materials and Methods Water Sampling and E.
All locations were sampled once and selected due to the presence of important discharges of treated or untreated wastewater into the river Table 1. San Pedro River is the main watershed and pluvial collector of Aguascalientes State.
Currently, the San Pedro River is being contaminated by the influx of wastewater from population centers, industrial activities, agricultural activities, and livestock which results in different levels of surface water quality.
Diagram of sample locations along the San Pedro River. Location and main sources of pollution of the 30 sampling locations studied.
Water samples were collected in 180 mL sterile glass bottles in triplicates. To isolate E.
For further identification, possible E. Isolates meeting the E. The E.
Oligonucleotides used in this study. Microbiological Analysis The amount of total bacteria was estimated by testing for mesophilic microorganisms using the pour plates technique 9215 B. Fecal contamination was determined by measuring total coliform 9221 C and fecal coliform 9221 E using the standard fermentation technique for the most probable number MPN; APHA, 1998.
Antimicrobial Susceptibility Testing Antimicrobial susceptibility test of 150 E. Isolates that were resistant to three or more antimicrobial agents were defined to have a multiple drug resistant MDR phenotype.