Active substances: Amoxicillin
Several plant species in the Canadian boreal forest show promise as sources of new antibiotics.
A new class of potential antibiotics has been recovered recently from buds of Populus balsamifera L. Lavoie et al.
Buds from this species were used frequently by First Nation populations as treatment for a range of health problems, from the common cold to diabetes Moerman.
The use of P. In a previous study, we showed that P.
We also isolated for the first time the Balsacones A, B and C and elucidated their structures. Finally, in this work, we investigate the action mechanism responsible for the antibacterial activity of BC and assess its cytotoxicity on human normal cells.
Isolation and Characterization of Methicillin-Resistant S. We identified S.
We determined the S. API Staph strips were left for 24 h before the results were read. The cultures were first inoculated on a non-selective plate for 18—24 h.
We collected each colony and then transferred the individual colonies to a 5-ml tryptic-soy broth. We then inoculated the Muller-Hinton agar plates.
After 15 min, we applied the disks having a fixed concentration of antibiotics to the plate surface. We measured the growth inhibition zone around each disk, and we related the diameter of the zone to the bacteria susceptibility.
The results of the disk diffusion test are expressed as susceptible, intermediate, or resistant. We used S. Compound and Bacterial Strains Lavoie et al.
Purity was confirmed by 1 H-NMR proton magnetic resonance spectroscopy used to determine the structure of a molecule. Increasing concentrations of BC diluted in methanol were then added.
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