Active substances: Amoxicillin
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On each plate, serial dilutions of an amoxicillin standard solution were included to construct a standard curve.
The mix was vortexed for 3 min before adding 10 mL of water and shaking for 30 min to extract amoxicillin. The samples were centrifuged at 10 000 rpm and the top hexane layer was removed.
Mobile phase A consisted of 0. The gradient was run at 0.
The UV spectrum was collected on a Waters photodiode array detector using the Waters Empower 2 data system. The amoxicillin levels were determined by comparing to a standard curve constructed with the corresponding peanut butter matrix spiked with amoxicillin.
Pharmacokinetics of amoxicillin in mice The pharmacokinetics of amoxicillin was analyzed in mice.
Water was given ad libitum. For each sample. The HPLC was run using 0. Results Design and formulation of peanut butter—based formulations of amoxicillin The goal of the project was to develop a novel formulation of amoxicillin that supplied both amoxicillin and nutrition.
Ideally it would be ready to use without further preparation, stable without refrigeration and able to be taken by young children without chewing.
In this study we tested two peanut butter—based matrices as the base for a child-friendly formulation of amoxicillin, which we named NutMox.
To our knowledge, until now, peanut butter has not been used to formulate pharmaceuticals and its suitability as a matrix for antibiotics has not been tested. We reasoned that the low water and high fat content of a peanut butter matrix would likely be beneficial to the stability of the pharmaceuticals it carried.
We tested each formulation at 50 mg amoxicillin per gram of matrix.
Quantifying amoxicillin level in peanut butter matrix Peanut butter is a high-fat substance, and the amoxicillin in NutMox needed to be extracted from peanut butter before being measured.
Based on this known solubility, we developed an aqueous extraction procedure that extracted amoxicillin from peanut butter matrices through a series of water dilutions and extractions see Materials and methods. Extracted samples were then analyzed by a colorimetric assay using hydroxylamine and ferric ammonium sulfate.
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Will it affect my contraception? Amoxicillin doesn't stop contraceptive pills working, including the combined pill or emergency contraception.
Look on the pill packet to find out what to do.
Will it affect my fertility? There's no firm evidence to suggest that taking amoxicillin will reduce fertility in either men or women.
However, speak to a pharmacist or your doctor before taking it if you're trying to get pregnant.