Active substances: Isotretinoin
Published 14 Jul Abstract The present paper demonstrates the increased photostability of isotretinoin when loaded in microemulsion.
The photodegradation of isotretinoin, in methanol and microemulsion formulation was studied under direct sun light.
Each experiment was performed in triplicate.
Cumulative corrections were made to obtain the total amount of ITTN permeated at each time interval. The average cumulative amount of drug that permeated per unit surface area of the skin was plotted versus time.
Besides the physicochemical properties, the chemical stability of the investigated drug in the vehicle plays a major role. During the observation period, the formulations were stored at room temperature in tubes to simulate patient usage conditions.
During stability studies, the samples were taken at fixed time intervals Patel et al.In no patient was the treatment of liver enzymes and serum lipids was detected.
Skin retention study Skin retention study was performed to analyze the content of the drug in the skin.
At the end of the in vitro skin permeation study, the skin samples were washed with water and methanol on both sides and carefully dried.
Then a defined amount of methanol was added to each piece of skin. Histopathological investigation The rat abdominal skin was mounted on Franz diffusion cell.
A piece of fresh excised untreated skin sample was used as control. Each section was dehydrated using graded solutions of ethanol and then embedded in paraffin wax.
Tissues were divided into small pieces and stained with hematoxylin and eosin. Animal care and handling throughout the experimental procedure were performed in accordance with the CPCSEA guidelines. White New Zealand rabbits weighing 2.
Results and discussion Development and evaluation of microemulsion The solubility of ITTN in various oils, emulsifiers and co-emulsifiers was analyzed to screen for components of ME.
It has been reported that the large solubilizing capacity of ME leads to larger concentration gradient toward the skin, providing superior dermal flux, for which high solubility of ITTN in oily phase can be an added advantage to increase it in ME.
Also, literature reveals that IPM acts as a chemical enhancer by disordering the prearranged lipids of the horny layer. The solubility of ITTN was determined in different emulsifiers to find a suitable emulsifier for the preparation of ME.